The tutorial of Sequences preprocessing

Step 1

Please Select Sequences preprocessing on the top bar.

Step 2

Select 1. Sequneces summary

In this step, we will summrize the sequences.


Step 3

Select the directory containing the sequences files and select the sequences type

Step 4

Click on the button Start!

Step 5

Click on the button View! to inspect the result.

If you want to know what the result looks like, you can click on the button Eaxmple for ….

Step 6

Click 2. Denoising on the top bar.

Step 7

Input the position number and quality number to trim the sequences
  1. Starting and ending position: If the starting position is 5 and the ending position is 120, it means the sequences would be retained from 5 to 120 bp. The sequences less than 120 bp would be discarded. As a result, you would get the about 115 bp sequences. If ending position is 0, no truncation or length filtering will be performed.
  2. Quality score: Reads are truncated at the position of a quality score less than or equal to this value. If the resulting read is then shorter than ending position, it is discarded.

Input the parameter of chimera, training error model, thread and upload the metadata
  1. Chimeric reads filter: Chimeric read is a sequence originate from multiple or parent sequences. They are generally considered a contaminant, as a chimera can be interpreted as a novel sequence while it is in fact an artifact.
  2. Training error model: The number of reads to use when training the error model. Smaller numbers will result in a shorter run time but a less reliable error model.
  3. Threads: Multiple threads to speed up the analytical process. The used thread is more, the required time is less.
  4. Integrating the metadata (Optional): If the metadata is provided, the results would have metadata information.